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A deposition was a first-person account given by a witness or participant in a matter undergoing investigation.

In 2267, after the apparent death of Benjamin Finney aboard the USS Enterprise, Captain Kirk gave a sworn deposition that he had jettisoned an ion pod with Finney onboard only after the ship went to red alert. Since the ship's log said otherwise, Commodore Stone accused Kirk of willful perjury. (TOS: "Court Martial")

In 2366, the destruction of the Tanuga IV research station and the death of Dr. Nel Apgar in a mysterious explosion was investigated by the Tanuga IV security force and the USS Enterprise-D. As part of the investigation, the parties involved gave depositions as to what happened in the events leading up to the explosion that were used to create holographic recreations. Commander William T. Riker's deposition was Deposition Program Riker One. Dr. Apgar's wife, Manua Apgar, used Manua Simulation One as her deposition while Dr. Apgar's assistant, Tayna used Tayna Simulation Three and Tayna Simulation Four for her depositions. (TNG: "A Matter of Perspective")

After Joshua Albert had been killed in an accident in 2368, Rear Admiral Brand had the other members of Nova Squadron give depositions in front of her and Captain Satelk. (TNG: "The First Duty")

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