At the age of sixteen, Culber was injured in a fall while hiking on the cliffs of Cabo Rojo, on Earth. Culber was rescued by a Doctor Kashkooli, who stitched and closed a puncture wound on his shoulder, which gave him a "very sexy scar.", as described by Paul Stamets. The experience inspired Culber to go to medical school. (DIS: "The Sound of Thunder")
In December of 2256, Specialist Michael Burnham introduced Culber to "Ripper", a tardigrade, after coming to believe that Ripper was experiencing pain during the starship's spore jumps. Culber agreed to run some tests. Scans of Ripper's frontal lobe showed significant cumulative deterioration every time Discovery went to black alert. The next time Discovery went to black alert, Ripper collapsed into a state of extreme cryptobiosis, reducing water content levels to one percent. Culber could barely detect Ripper's vital signs, and neurological tests indicated Ripper was possibly sentient. Acting Captain Saru ordered Culber to re-hydrate Ripper for more spore jumps. Culber refused the order, stating that he would "not be party to murder." Saru then ordered Lieutenant Stamets to carry out the order. Unbeknownst to Commander Saru, Lieutenant Stamets injected himself with Ripper's DNA and operated the spore drive in place of Ripper. Stamets rationalized to Culber that he knew he would leave him if he allowed anything else to happen to Ripper. (DIS: "Choose Your Pain")
After Ash Tyler experienced nightmares of having undergone surgery following Klingon imprisonment, he asked Culber to perform an examination on him. Culber discovered that Tyler's skeletal structure and organs had been surgically altered and that the Tyler personality had been overlaid over that of someone else. This led him to try to ground Tyler, which triggered a reaction in Tyler, who broke Culber's neck, killing him. (DIS: "Despite Yourself") It was later discovered that Culber had been correct and that Ash Tyler's personality had overlaid that of Voq, a Klingon. (DIS: "The Wolf Inside")
After his resurrection, Culber was subjected to extensive scans. (DIS: "Saints of Imperfection") Doctor Tracy Pollard ran every scan or test she could think of on Culber, to the point that Culber thought there wasn't a scan or test that she had not performed. While the scans showed his new body was healthy, Culber's body was completely new, with even the old scar on his shoulder gone. Pollard advised Culber that it would take time for him to get used to his new body and that, while she would continue to monitor his condition, he could resume a normal life. (DIS: "The Sound of Thunder")
Culber decided to transfer to the USS Enterprise, but he eventually changed his mind, when he realized that his place was with Stamets on the Discovery. He was among multiple crewmembers who volunteered to stay on board when Burnham took the ship forward into the 32nd century, to elude Control, with no chance of coming back. Along with the rest of the volunteering crew, Culber was presumed dead by Starfleet, based on the testimonies of the Enterprise senior officers. (DIS: "Such Sweet Sorrow", "Such Sweet Sorrow, Part 2")
After Discovery arrived in the year 3189, Culber helped the crew adjust to their new lives in a time in which everyone they had known had died centuries earlier. In particular he helped Keyla Detmer deal with her guilt at being the one who piloted Discovery through the wormhole to the future. (DIS: "Forget Me Not")
During an investigation into the Burn, Culber was beamed down to wreckage of the KSF Khi'eth in the Verubin Nebula with Burnham and Saru. During this time, a holoprogram disguised him as a Bajoran in an outfit that hid his medical supplies and away mission gear. When Osyraa attacked the USS Discovery, he elected to stay behind with Saru and Su'Kal on the dilithium nursery they were on. (DIS: "Su'Kal")
- "The Butcher's Knife Cares Not for the Lamb's Cry" (First appearance)
- "Choose Your Pain"
- "Magic to Make the Sanest Man Go Mad"
- "Si Vis Pacem, Para Bellum"
- "Into the Forest I Go"
- "Despite Yourself"
- "The Wolf Inside" (body)
- "Vaulting Ambition"
- "What's Past Is Prologue" (archive footage)
- "Brother" (holographic recording)
- "Saints of Imperfection"
- "The Sound of Thunder"
- "If Memory Serves"
- "The Red Angel"
- "Perpetual Infinity"
- "Through the Valley of Shadows"
- "Such Sweet Sorrow"
- "Such Sweet Sorrow, Part 2"
- "Far From Home"
- "Forget Me Not"
- "Die Trying"
- "The Sanctuary"
- "Terra Firma, Part 1"
- "Terra Firma, Part 2"
- "That Hope Is You, Part 2"
Culber is portrayed by Wilson Cruz. He stated about the character, "It was important for me, when we were developing Doctor Culber, that he had this big heart, but he was also someone who was formidable, who could stand up to authority, who, you know, had no problem voicing his opinions and insisting on going the right way. So, I think we achieved that." (AT: "Despite Yourself")