(covers information from several alternate timelines)
"It's not every day you meet the girl you're going to marry."
"Do you use this routine a lot with women?"
"No, never before and never again."
Jennifer met Ben on Gilgo Beach in 2354, the day after attending a party at her friend George's place. They were married soon after. Later that year, Jennifer and Benjamin attended the Mazurka Festival on New Berlin with Cal and Gretchen Hudson. (DS9: "Emissary", "The Maquis, Part I")
Prior to 2367, the Sisko family made a camping trip to Itamish III, where Jake learned water skiing. While on a camping trip on a planet in the Gamma Quadrant in 2370, Jake recalled this vacation as the happiest time he and his parents had had together. (DS9: "The Jem'Hadar")
Jennifer and Jake accompanied Ben on his assignment to the USS Saratoga, where she was killed when that ship was destroyed by the Borg at the Battle of Wolf 359 in 2367. Jennifer's death devastated Ben and it was years before he began to move on emotionally. In fact, his bitterness and anger over her death, specifically toward Captain Jean-Luc Picard, whom the Borg assimilated and forced to lead their invasion of Earth as Locutus, almost led him to resign his Starfleet commission, before he accepted that he was trying to escape his memories of Jennifer's death rather than Starfleet itself. (DS9: "Emissary", "The Way of the Warrior")
Jennifer's death haunted Ben for years. He had trouble sleeping around the fourth anniversary of her death. Shortly after, he met Fenna, who he told Jadzia Dax was the first person he'd "felt really drawn to" since Jennifer's death. (DS9: "Second Sight")
Jennifer Sisko was played by actress Felecia M. Bell.
In Voyages of Imagination, Robert Simpson notes that he and Marco Palmieri pitched a story to the DS9 writers that featured the return of Jennifer Sisko. Their idea was to have it revealed Jennifer was taken from the Saratoga by the Borg and assimilated. Benjamin Sisko would then encounter her. The pitch never sold, although Simpson later used the idea in the Mission Gamma novel Lesser Evil for the wife of DS9's new XO Elias Vaughn.
The novel The Needs of the Many gave her the middle name "Felecia".
According to the Star Trek: The Lost Era novel Deny Thy Father, Jennifer was in labor for twenty hours before Jake was born. The birth took place in San Francisco on Father's Day in June 2355. Benjamin Sisko was on the paternity leave from the USS Livingston at the time and was able to attend the delivery.