Nitrous oxide (N2O) is a colorless gas that often made Humans insensible to pain and caused spontaneous laughter; due to this effect, it was often dubbed laughing gas.

In 2270, when the computer circuits of the USS Enterprise were affected by subatomic particles from an energy field encountered by the ship and began playing practical jokes on the crew, among its tricks was to fill the ship with nitrous oxide.

After a few minutes of exposure to nitrous oxide, a Vulcan would loss consciousness, as their physiology was unable to process the effects of the gas. (TAS: "The Practical Joker")

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