(written from a Production point of view)
The first volume of six issues began its release in early October 2007. Three of the stories were set in the Star Trek: The Original Series time frame and three during the Star Trek: The Next Generation era. An omnibus edition was released 14 May 2008 collecting all six issues.
A second volume was later announced. Originally expected to be released in summer 2008, it eventually saw release beginning in March 2009. The species which received focus in this volume are the Klingons, Cardassians, the Romulans, the Q, and Tribbles. Andrew Steven Harris was originally announced as writing a Ferengi-centric issue,  while James Patrick and Mike W. Barr were also announced as initially contributing, although their species assignments were never announced. 
- Based on Star Trek created by Gene Roddenberry.
- Interior Artist:
- "Alien Spotlight: The Gorn"
- "Alien Spotlight: Vulcans"
- "Alien Spotlight: Andorians"
- "Alien Spotlight: Orions"
- "Alien Spotlight: Borg"
- "Alien Spotlight: Romulans"