Starbase 133 was a Federation starbase administered by Starfleet. The Spacedock-type space station orbited a planet. Dr. Dalen Quaice worked at Starbase 133 for six years, from 2361 to 2367. He retired from his post after the death of his wife Patricia Quaice.

In 2366, after completing its mission to investigate the disappearance of the colony on Rana IV, the USS Enterprise-D departed for Starbase 133. (TNG: "The Survivors")

A year later, Starbase 133 was the site of an Enterprise-D crew rotation. The ship also picked up Dr. Quaice in order to take him back to his home on Kenda II. While docked at the starbase, an experiment conducted by Wesley Crusher trapped Dr. Beverly Crusher in an alternate universe, contained within a warp bubble. One day later, the Enterprise was returned to its exact position within the starbase during the accident in order to create a gateway for Dr. Crusher to return. (TNG: "Remember Me")

Shots of Starbase 133 were reused footage of Starbase 74 from "11001001", with the exterior shots in turn being reused footage of the Earth Spacedock from Star Trek III: The Search for Spock.

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