This page contains information regarding Star Trek: Lower Decks, and thus may contain spoilers.
For generations, the starbase hosted the annual Command Conference and corresponding afterparty. Sometime during the 2260s, James T. Kirk and Spock visited this station for the conference, but failed to gain entry to the conference's afterparty. The two ended up in a nearby dive bar, where they (or at least Kirk) drank the night away, eventually leaving their names behind, carved into the bartop. (LD: "An Embarrassment Of Dooplers")
In 2269, the shuttlecraft Copernicus, from the USS Enterprise, was transporting a Slaver stasis box to the base, when it was lured into the Beta Lyrae system by Kzinti privateers. (TAS: "The Slaver Weapon")
In 2381, the USS Cerritos visited the base when it escorted a Doopler emissary to the station. Their arrival coincided with that year's Command Conference, which was held aboard the station. The station's commander was Captain John Anderson, who upon observing the Doopler emissary the Cerritos was escorting to the base for trade negotiations begin to "Dooplercate", he denied the Cerritos permission to dock until they sorted the problem out. (LD: "An Embarrassment Of Dooplers")
The massive space station was located in orbit of a nearby planet, and was capable of facilitating a large number of starships at once. It was considered to be quite old by the 2380s. (LD: "An Embarrassment Of Dooplers")
Businesses on the station's multi-level promenade included, the aforementioned Quark's Bar, Malvus' Goods, The Lucky Ferengi, Mr. Krada Leg, and Ballroom Alpha, as well as a number of unspecified business, such as a casino, a barbershop, a fine men's clothing store, and an aviary. (LD: "An Embarrassment Of Dooplers")
Station security was a civilian law enforcement agency that patrolled the station with karts, and with that, "terrible drivers". According to Beckett Mariner, "station security has a rep for being crooked," fearing that if they were to be arrested by them "[t]hey aren't gonna let us off with a warning and a glass of Thai iced tea. They'll coerce a confession, then sell our combadges on the black market." It was further noted that "[t]hey're always amped up on party day"– the day of the Command Conference afterparty. (LD: "An Embarrassment Of Dooplers")
According to the revised Stellar Cartography: The Starfleet Reference Library (Alpha Quadrant: Local Space Extract), Starbase 25 was located in the Alpha Quadrant.
The design of the station resembles Starbase 47 from the Star Trek: Vanguard novel series.